Bioinformatics-based analysis of the bZIPs.

<p><b>A)</b> Prediction of the coiled coil (CC, blue) and LZ (LZ, red) motifs for the bZIP domains of the 16 bZIP proteins by the 2ZIP software. The heptads are numbered based on Deppmann <i>et al</i>. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0139884#pone.0139884.ref019" target="_blank">19</a>]. Predictions from the COILS program are found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0139884#pone.0139884.s002" target="_blank">S2 Fig</a>. <b>B)</b> Electrostatic interactions calculated between pairs of bZIPs. The numbers indicate the attractive—repulsive interactions between <b>e</b>-<b>g</b> positions. Interactions that lay outside of the predicted coiled coil region in at least one of the monomers are indicated in parenthesis. Combinations with a positive balance between attractive and repulsive electrostatic interactions are indicated in blue, negative in red, and neutrally balanced in black. The background is colored according to the different bZIP networks. The number of interactions was calculated with DrawCoil 1.0 (<a href="http://www.grigoryanlab.org/drawcoil" target="_blank">www.grigoryanlab.org/drawcoil</a>). <b>C)</b> Charge-hydropathy plot to predict protein disorder, in which the line represents the boundary between disorder (above) and order (below). <b>D)</b> Cumulative Distribution Functions on the PONDR VL-XT algorithm are shown for C-bZIPs (blue), S1-bZIPs (red), G-bZIPs (green), and H-bZIPs (yellow). The more concave the line is below the boundary represented by the dotted line, the more disordered the protein. Conversely, the more convex it is above the boundary, the more ordered. In C) and in D) the sequence analyzed spans from heptad 0 to heptad 5 in H-bZIPs, to heptad 7 in G-bZIPs, and to heptad 10 in C- and S1-bZIPs.