Inhibition of Hela cells proliferation by CpG loaded particles and doxorubicin hydrochloride (DOX).

<p>RAW264.7 and Hela were placed in the upper and lower chambers of Transwell plates, respectively. CpG loaded complexes and DOX were added to the upper side at a final concentration of 10 μg CpG/ml and 1 μg DOX/ml. The proliferative activity of Hela cells was measured at 48 h with the MTT assay and was converted to a percentage of the medium-treated (control) cells. Results are expressed as mean±SD of three independent measurements. CpG (and CpG loaded particle) group displayed significant differences, compared to non-CpG controls. CpG (and CpG loaded particles) also present different cell viabilities compared to the DOX treatment group (**<i>P</i><0.01).