Additional file 15: Figure S7. of Secretome profiling of Cryptococcus neoformans reveals regulation of a subset of virulence-associated proteins and potential biomarkers by protein kinase A

PCR to confirm absence of cellular factors due to cell lysis in secretome of C. neoformans. PCR for the A) Actin and B) PKA1 genes were performed using either C. neoformans WT genomic DNA (6 pg to 100 ng) or WT supernatant samples (250 ng or 1000 ng of total protein). Control lane contains 6 pg of gDNA spiked into 250 ng of supernatant. (TIFF 1093 kb