基于AFM的药物刺激前后淋巴瘤活细胞的形貌及弹性的变化

Atomic force microscopy(AFM) provides a means for characterizing the surface topography and biophysical properties of individual living cells under near-physiological conditions.However,owing to the lack of adequate cellular immobilization methods,AFM imaging of living,suspended mammalian cells is still a big challenge.In this paper,a method is presented for immobilizing individual living B lymphoma cells that combines mechanical trapping with pillar arrays and electrostatic adsorption with poly-L-lysine.In this way,the topography and elasticity changes of individual B lymphoma cells that were stimulated with different concentrations of Rituximab were observed and measured dynamically.When the cell is stimulated by 0.2 mg·mL-1Rituximab for 2 h,the cell topography becomes more corrugated and Young smodulus decreases from 196 to 183 kPa.When the cell is stimulated by 0.5 mg·mL-1Rituximab for 2 h,thecell topography changes more significantly and some tubercles appear,and Young s modulus decreasesfrom 234 to 175 kPa.These results thus provide a unique insight into the effects of Rituximab on individualcells.