The number of ciliated MDCK cells after seeding increases over time.

<p><b>(A)</b> Cells were fixed at respective time points and stained with antibodies against acetylatedTubulin for cilia (magenta), Cep164 for the mother centriole (green) and Hoechst for nuclei (blue). Arrows point to mother centrioles which do not have cilia. <b>(B)</b> The number of ciliated cells was quantified over time: 6h: 0.0% ±0.0%, 3420 cells; 1d: 0.0% ±0.0%, 9347 cells; 2d: 0.1% ±0.1%, 18600 cells; 3d: 3.5% ±1.7%, 18309 cells; 7d: 43.2% ±4.7%, 17418 cells and 10d: 51.9% ±3.6%, 17475 cells. n = 3 (32 fields of view per N).