Ift88 expedites cell migration in ciliated MDCK cells.

<p><b>(A)</b> Ift88-i1 cells were grown to confluency for 7–8 days (ciliated stage) and subjected to wounding. Cells depleted of Ift88 by inducible shRNA (+Tet) migrate slower compared with non-induced control cells (-Tet). The leading edge is shown after 2h and 6h. Scale bars: 100μm. <b>(B)</b> Quantification of migration speed in different cell lines expressing different tetracycline inducible shRNAs. No significant (n.s.) reduction in migration speed is observed in cells expressing unspecific shRNA (control; -Tet: 111.5 ±5.8 μm²/minute vs. 105.3 ±10.2 μm²/minute, p = 0.46, n = 4). Two independent cell lines expressing different shRNAs against Ift88 show significantly reduced migration speed: Ift88-i1: -Tet: 111.0 ±4.7 μm²/minute vs. +Tet: 64.6 ±10.3 μm²/minute, p<0.01, n = 4; Ift88-i2: -Tet: 124.7 ±1.2 μm²/minute vs. +Tet: 56.7 ±6.8 μm²/minute, p<0.01, n = 3. Migration speed is partially restored in Ift88-i1 cells expressing non-degradable Ift88 mRNA (Ift88-i1.rescue): -Tet: 116.5 ±5.5 μm²/minute vs. +Tet: 85.8 ±4.3 μm²/minute, p<0.01, n = 4.