Coat mislocalization in the absence of SpoIIQ and SpoIIIA proteins.

<p>DIC and fluorescence microscopy of WT, <i>spoIIQ</i>^<i>−</i>(<i>IIQ</i>^<i>–</i>), <i>spoIIIA</i>^{<b><i>−</i></b>}(<i>IIIA</i>^<i>–</i>), <i>spoIIIAH</i>^{<b><i>−</i></b>}(<i>IIIAH</i>^<i>–</i>), <i>sigG</i>^<i>–</i>, and <i>spoIVA</i>^<i>−</i>(<i>IVA</i>^<i>–</i>) strains producing CotE-SNAP fusions. Cells were collected after 24 hrs on sporulation media and labeled with the SNAP substrate TMR-Star (green), the lipophilic dye FM4-64 (red), and the Hoechst nucleoid dye (blue). Yellow arrows indicate where the SNAP signal (green) overlaps with FM4-64 staining (red). Scale bars represent 2 μm.