Morphological defects of <i>spoIIQ</i>, <i>spoIIIA</i>, and <i>spoIIIAH</i> mutants.

<p>Transmission electron microscopy (TEM) of WT, <i>spoIIQ</i>^<i>−</i>(<i>IIQ</i>^<i>–</i>), <i>spoIIIA</i>^{<b><i>−</i></b>}(<i>IIIA</i>^<i>–</i>), <i>spoIIIAH</i>^{<b><i>−</i></b>}(<i>IIIAH</i>^<i>–</i>), <i>sigG</i>^<i>–</i>, and <i>spoIVA</i>^<i>−</i>(<i>IVA</i>^<i>–</i>) strains grown for 24 hrs on sporulation media. The forespore region of these strains is shown on the right. Black arrows indicate regions that resemble coat layers surrounding the forespore. White arrows indicate coat that appears anchored to the leading edge of the engulfing membrane but is not intimately associated with the mother cell-forespore interface. Yellow arrows highlight coat that appears to be mislocalized away from the forespore region to the mother cell cytosol. Scale bars represent 500 nm.