Reduced levels of phosphorylated STMN1 in FANCC mutant cells.

<p>(A) Western blot analysis of protein extracts from FANCC mutant (PD331) and FANCC-corrected (PD331/C) cells. Cells were unsynchronized (UN) or synchronized (S) using nocodazol (10 μM for 16 hours). Total protein extracts were immunoblotted (IB) with the indicated antibodies. Arrows indicate total STMN1 and STMN1 phosphorylated species. (B) Graph displaying the mean relative ratio of STMN1/γ-tubulin protein expression from 3 separate experiments. (C) Western blot analysis of protein extracts from cells overexpressing serine-to-alanine Myc-tagged STMN1 mutants. Total protein extracts were immunoblotted (IB) with the indicated antibodies