Expression of active Akt is downregulated in diabetic mice kidneys.

<p>Immunofluorescence staining of the control, stz injected diabetic mice for Akt-ser473 (Cell Signaling) was performed. Kidneys were double stained with anti-Rabbit FITC. Mouse kidney with knockout of Akt1 (Akt1KO) was utilized as a negative control. Immunofluorescence imaging revealed that phosphorylation of Akt at the 273 serine residue was diminished in diabetic mice kidneys conforming decrease activation of Akt in diabetic animal kidneys before occurrence of significant changes due to T1D.