<i>Plasmodium chabaudi adami</i> selectivity for red blood cells.

<p>At day 6 and 7 post- <i>P</i>. <i>c</i>. <i>adami</i> infection, thin blood smears of parasite ring stages iRBCs were fixed and stained with DAPI (blue). DIC and fluorescence images were captured with Nikon A1 confocal microscope (plan Apo VC 60x, NA 1.4, λs oil immersion), and analysed with NIS-Elements Viewer 4.20 imaging software. Single and multiple-iRBCs were counted in >150 ring-iRBCs per mouse (A, C). Parasite SI was calculated for each day by dividing the observed value of multiple iRBCs by the value expected from a Poisson distribution (B, D). DIC, DAPI fluorescence, and merged channels are shown at day 6 post-infection during ring stage for a control and a HE-treated mouse (E). The results represent the means ± SEM of one experiment (Ctrl; n = 4, HE; n = 3) at day 6 post-infection and two experiments (Ctrl; n = 8, HE; n = 7) at day 7 post-infection. Data were compared with an unpaired Student <i>t</i> test, **p<0.01, ***p<0.001.