<i>Plasmodium chabaudi adami</i> schizogony and invasion stage.

<p>Six days post-infection, thin blood smears of iRBCs were prepared at the time of schizogony and stained with DAPI (blue). DIC and fluorescence images were captured with Nikon A1 confocal microscope (objective plan Apo VC 60x, NA 1.4, λs oil immersion), and analysed with NIS-Elements Viewer 4.20 imaging software. The proportion of merozoites (nuclei) per mature schizont (containing ≥4 merozoites) was determined in >150 iRBCs per mouse (A). DAPI-stained blood smears were also analysed at the time of merozoite invasion, and the proportion of extra-erythrocytic merozoites was measured in ≥600 merozoites (number of extra-erythrocytic merozoites/total number of merozoites) (B). DIC, DAPI fluorescence and merged images at the time of merozoite invasion are shown for a control and HE-treated mouse (C). The results represent the mean ± SEM of one experiment (Ctrl; n = 4, HE; n = 3) and were compared with an unpaired Student <i>t</i> test, *p<0.05.