Changes in Gene and Protein Expression Induced in CBSC after Culture with Resting or Activated CB NK Cells.

<p>(<b>A and B</b>) CBSC were cultured for 4 h either alone or with resting or activated CB NK cells and then CBSC were re-isolated by cell sorting based on CD34 expression (N = 3). Gene expression profiles were compared by microarray analysis using GeneChip human Gene 2.0 ST Arrays and normalized. Data is presented as a Venn diagram and Volcano plots comparing the number of CBSC genes for which expression was changed (either up or down-regulated) by greater than 1.5 fold (p ≤ 0.05) after culture with resting or activated CB NK cells in comparison to CBSC alone (untreated). Refer to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138623#pone.0138623.s001" target="_blank">S1 Table</a> for specific changes in gene expression. (<b>C</b>) Validation of microarray data showing increased CXCL-9 expression by CBSC after culture with activated CB NK cells. CXCL-9 protein was measured in culture supernatants using ELISA after co-culture of CBSC alone or with resting or activated CB NK cells (N = 4). (<b>D</b>) CFU assays showing number of colonies formed after 4 h culture of CBSC with different concentrations of CXCL9 as indicated (N = 4), * P < 0.05.