SIRT1 deacetylates hnRNP L and hnRNP C1/C2 <i>in vivo</i>.

<p>Acetylated proteins were immunoprecipitated using the pan-acetyl-lysine antibody from the liver extracts of SIRT1^+/+ and <i>Sirt1</i>^<i>-/-</i> mice. The amounts of acetylated hnRNP L and hnRNP C1/C2 proteins were evaluated by their corresponding antibodies in the IP samples. The protein levels of hnRNP L, hnRNP C1/C2 and SIRT1 were assessed by western blotting using the corresponding antibodies. Histone H2A was used as a loading control.