Suppression of IL-1Ra expression using siRNA.
- Hisashi Goto (814821)
- Yuichi Ishihara (814822)
- Takeshi Kikuchi (328786)
- Ario Izawa (814823)
- Nobuaki Ozeki (482377)
- Eijiro Okabe (814824)
- Yosuke Kamiya (814825)
- Yusuke Ozawa (645073)
- Hiroki Mizutani (814826)
- Genta Yamamoto (814827)
- Makio Mogi (482378)
- Kazuhiko Nakata (482382)
- Hatsuhiko Maeda (814828)
- Toshihide Noguchi (63609)
- Akio Mitani (814829)
Publication date
October 2015
DOIAbstract
<p>(A) Determination of IL-1Ra mRNA levels using real-time PCR. At 48 hours after transfection, IL-1Ra siRNA-transfected cells showed clear knockdown of IL-1Ra mRNA compared with the control. Differences among groups were analyzed using the Student’s <i>t</i>-test. Data are expressed as the mean ± SD (n = 6). **<i>p</i> < 0.01 vs control. (B) Western blot of IL-1Ra (18 KDa) in cells transfected with either IL-1Ra or control siRNAs (n = 3). Data are representative of three independent experiments.</p
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