The sequence of the <i>braB</i> regulatory region and map of the promoter fragments used.

<p>A. The sequence (5’ to 3’) of the coding (non-template) strand of the <i>braB</i> regulatory region within the <i>braB242-lacZ</i> fusion. Coordinates are reported with respect to the transcription start point. The upstream boundary of the <i>braB184</i>, <i>braB162</i>, and <i>braB144</i> fusions at positions –87, -65, and -47, respectively, are indicated by vertical arrows above the sequence. The vertical arrows below the sequence indicate the junction points, at position +11 and +36, between the <i>braB</i> and <i>lacZ</i> sequences. The likely translation initiation codon, -10 and -35 promoter regions, and apparent transcription start point are in boldface. The directions of transcription and translation are indicated by the horizontal arrows. The sequences on the template strand that were protected by CodY or ScoC in DNase I footprinting experiments are underlined or shown by dotted horizontal lines below the sequence, respectively. The sequences of CodY-binding motifs are italicized and shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005600#pgen.1005600.t001" target="_blank">Table 1</a>. The mutated nucleotides are shown in lowercase above the sequence. B. Schematic maps of the <i>braB</i> fragments used to construct <i>lacZ</i> fusions or in DNA-binding experiments. The coordinates indicate the boundaries of different fusions with respect to the <i>braB</i> transcription start point. The location of the apparent transcription start point is indicated by the bent arrow. CodY- and ScoC-binding sites determined in DNase I footprinting experiments are shown as clear or shaded rectangles, respectively.