Up-regulated PRA–target genes in the presence of P4.

<p>MDA-iPRAB cells were cultured during 24 h in steroid-free medium containing RSL1 (0.5 μM) and/or Dox (2 μM) to induce expression of PRA and/or PRB. Cells were treated by vehicle or P4 (1 nM) for 6 hours and a genome wide transcriptomic studies was performed as described in [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140795#pone.0140795.ref006" target="_blank">6</a>]. A total of 999 genes were affected by PR expression. These genes were separated into three groups: A and B and AB (co-expressed at similar levels relative to physiological conditions) regrouping genes that are regulated by either by PRA, PRB or PRAB. Tables above represent class <i>A+</i> genes regulated by PRA in the presence of the ligand; Class <i>A+&AB+</i>, genes responsive to liganded PRA and their expression may or may not be influenced by PRB co-expression; Class <i>A±&AB±</i> genes responsive to both liganded and unliganded PRA, and their expression may be influenced by PRB co-expression; Class <i>A+&B+& AB+</i>, genes that are commonly regulated by liganded PRA, PRB and PRAB. FC calculated either by microarray studies or by RT-qPCR analysis and their function in the cancer. FC: Transcriptional Fold Change calculated by microarray studies (FC*) and by RT-qPCR (FI, Fold Induction).</p><p>Up-regulated PRA–target genes in the presence of P4.