Hi3 peptide quantitative analysis of abundant plasma proteins.

<p><b>Median (ng/mL)</b>: median of protein concentrations determined from number of samples shown in column <b>n</b>. <b>IRV</b>: inter-assay variation, the coefficients of variation obtained from 7 aliquots of a pooled sample separately digested and measured over the course of 3 months of normal operation of the instrument. <b>IAV:</b> intra-assay variation, coefficient of variation obtained from 6 aliquots of a pooled sample separately digested and subsequently measured during 1 day. <b>AV</b>: analytical variability, determined from 10 replicate injections of a single digested sample throughout 9 days of measurements. <b>LIN:</b> linearity of measurements, the Pearson’s correlation coefficient shows linearity between total protein load on the analytical column and nanogram protein quantified by HI3 peptide quantitation using the ADH1 digest standard; only determined when at least 4 points were available for a protein. See also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140097#pone.0140097.s001" target="_blank">S1 Fig</a>. <b>ST:</b> Pearson’s correlation of protein quantitation using two different digest standards (ADH1 and ENO1 from yeast). <b>LOQ:</b> limit of quantitation (ng/mL) estimated by dilution of a plasma sample in a constant background of a digest standard. <b>n:</b> number of samples (out of 31) in which the protein was quantified, <b>FN:</b> protein number on the x-axis of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140097#pone.0140097.g001" target="_blank">Fig 1c</a>. <b>hc:</b> heavy chain.</p><p>(50:1 substrate:enzyme, 20 hours digestion, 0.21 ug on column injection).