Detection of GFP protein in sugar-starved transgenic rice suspension cell cultures.

<p><b>A,</b> Rice suspension cells (cell volume of 0.5 mL) were cultured in 1 mL of sugar-free MS medium for 2 and 3 days. Total cellular soluble proteins were isolated in 1 mL of protein extraction buffer from each of the treated cell cultures. Twenty microliters of total cellular protein (C) and 20 μL of total medium protein (M) were subjected to Western blot analysis using GFP antibodies. <b>B,</b> The proportion of medium GFP protein in various transgenic suspension cell lines. The amount of GFP within cells and medium was detected, and converted to the total amount of GFP protein. The proportion of medium GFP in each independent line is presented as the total GFP in the medium compared with the total GFP. A dark gray bar indicates medium GFP, while a light gray bar indicates cellular GFP. Error bars indicate the standard deviation of three Western blot replicates from two biological repeats.