<i>dFoxO</i> is required for loss-of-<i>pll</i> induced wing phenotypes.

<p>(<b>A</b>-<b>R</b>) Light micrographs showing <i>Drosophila</i> adult wings, anterior is to the left and distal up. Compared with controls (<b>A</b>, <b>G</b> and <b>M</b>), the wing phenotypes of <i>ptc</i>><i>pll-IR</i>^<i>V2889</i> (<b>B</b>), <i>Sd>pll-IR</i>^<i>V2889</i> (<b>H</b>) and <i>Omb>pll-IR</i>^<i>V2889</i> (<b>N</b>) flies were suppressed by knocking-down <i>dFoxO</i> (<b>D</b>, <b>J</b> and <b>P</b>) or removing one or both copies of <i>dFoxO</i> (<b>E</b>, <b>F</b>, <b>K</b>, <b>L</b>, <b>Q</b> and <b>R</b>), but not by expressing a <i>GFP-IR</i> (<b>C</b>, <b>I</b> and <b>O</b>). In <b>A</b>-<b>F</b>, the lower panels are high magnification of the boxed areas in upper panels. (<b>S</b>-<b>U</b>) Statistical analysis of the ACV phenotype (<b>S</b>) and the adult wing size/wild type (WT) (<b>T</b> and <b>U</b>) as shown in figures <b>A</b>-<b>F</b>, <b>G</b>-<b>L</b> and <b>M</b>-<b>R</b> respectively. One-way ANOVA with Bonferroni multiple comparison test was used to compute <i>P</i>-values, significance is indicated with asterisks (*** <i>P</i><0.001). ns stands for not significant. Detailed genotypes: (A) <i>ptc-</i>Gal4/<i>+</i> (B) <i>ptc-</i>Gal4/<i>UAS-pll-IR</i>^<i>V2889</i> (C) <i>ptc</i>-Gal4/<i>UAS-pll-IR</i>^<i>V2889</i>/+; <i>UAS-GFP-IR</i>/+ (D) <i>ptc</i>-Gal4/<i>UAS-pll-IR</i>^<i>V2889</i>/+; <i>UAS-dFoxO-IR</i>/+ (E) <i>ptc</i>-Gal4/<i>UAS-pll-IR</i>^<i>V2889</i>/+; <i>dFoxO</i>^Δ94/+ (F) <i>ptc</i>-Gal4/<i>UAS-pll-IR</i>^<i>V2889</i>/+; <i>dFoxO</i>^Δ94/Δ94 (G) <i>Sd-</i>Gal4/<i>+</i> (H) <i>Sd-</i>Gal4/<i>+</i>; <i>UAS-pll-IR</i>^<i>V2889</i>/+ (I) <i>Sd-</i>Gal4/<i>+</i>; <i>UAS-pll-IR</i>^<i>V2889</i>/+; <i>UAS-GFP-IR</i>/+ (J) <i>Sd-</i>Gal4/<i>+</i>; <i>UAS-pll-IR</i>^<i>V2889</i>/+; <i>UAS-dFoxO-IR</i>/+ (K) <i>Sd-</i>Gal4/<i>+</i>; <i>UAS-pll-IR</i>^<i>V2889</i>/+; <i>dFoxO</i>^Δ94/+ (L) <i>Sd-</i>Gal4/<i>+</i>; <i>UAS-pll-IR</i>^<i>V2889</i>/+; <i>dFoxO</i>^Δ94/Δ94 (M) <i>Omb-</i>Gal4/<i>+</i> (N) <i>Omb-</i>Gal4/<i>+</i>; <i>UAS-pll-IR</i>^<i>V2889</i>/+ (O) <i>Omb-</i>Gal4/<i>+</i>; <i>UAS-pll-IR</i>^<i>V2889</i>/+; <i>UAS-GFP-IR</i>/+ (P) <i>Omb-</i>Gal4/<i>+</i>; <i>UAS-pll-IR</i>^<i>V2889</i>/+; <i>UAS-dFoxO-IR</i>/+ (Q) <i>Omb-</i>Gal4/<i>+</i>; <i>UAS-pll-IR</i>^<i>V2889</i>/+; <i>dFoxO</i>^Δ94/+ (R) <i>Omb-</i>Gal4/<i>+</i>; <i>UAS-pll-IR</i>^<i>V2889</i>/+; <i>dFoxO</i>^Δ94/Δ94.