Loss of <i>pll</i> produces Toll/NF-κB pathway independent wing phenotype.

<p>(<b>A</b>-<b>L</b>) Light micrographs showing <i>Drosophila</i> adult wings, anterior is to the left and distal up. Compared with <i>ptc</i>-Gal4 control (<b>A</b>), expression of <i>pll</i> RNAi (<i>pll-IR</i>^<i>V2889</i>) induced a loss-of-ACV phenotype (<b>C</b>) that was rescued by expression of Pll (<b>D</b>), while expression of Pll alone showed no obvious defects (<b>E</b>). Expression of RFP was included as a negative control (<b>B</b>). Down-regulation of Toll/NF<b>-</b>κB pathway by expressing RNAi of <i>Toll</i> (<b>F</b>), <i>tube</i> (<b>G</b>), <i>dorsal</i> (<b>I</b>) or <i>Dif</i> (<b>J</b>), or the negative regulator Cactus (<b>H</b>), had no effect on wing development. <i>ptc>pll-IR</i>^<i>V2889</i> was not rescued by co-expressing a <i>cactus</i> RNAi (<b>K</b> and <b>L</b>). The lower panels show high magnification view of the ACV area, boxed in panel A, in upper panels. (<b>M</b>) Quantification of the ACV phenotype as shown in figures <b>A</b>-<b>L</b>. One-way ANOVA with Bonferroni multiple comparison test was used to compute <i>P</i>-values, significance is indicated with asterisks (*** <i>P</i><0.001). ns stands for not significant. Detailed genotypes: (A) <i>ptc</i>-Gal4/+ (B) <i>ptc</i>-Gal4/+; <i>UAS</i>-RFP/+ (C) <i>ptc</i>-Gal4/<i>UAS-pll-IR</i>^<i>V2889</i> (D) <i>ptc</i>-Gal4/<i>UAS-pll-IR</i>^<i>V2889</i>; <i>UAS</i>-Pll; (E) <i>ptc</i>-Gal4/+; <i>UAS</i>-Pll/+ (F) <i>ptc</i>-Gal4/+; <i>UAS-Toll-IR</i>/+ (G) <i>ptc</i>-Gal4/+; <i>UAS-tube-IR</i>/+ (H) <i>ptc</i>-Gal4/<i>UAS</i>-Cactus (I) <i>ptc</i>-Gal4/+; <i>UAS-dorsal-IR</i>/+ (J) <i>ptc</i>-Gal4/+; <i>UAS-Dif-IR</i>/+ (K) <i>ptc</i>-Gal4/+; <i>UAS-cactus-IR</i>/+ (L) <i>ptc</i>-Gal4/<i>UAS-pll-IR</i>^<i>V2889</i>; <i>UAS-cactus-IR</i>/+.