Resistance phenotypes caused by loss of Hat1 are specific for <i>C</i>. <i>albicans</i>.

<p>(A) Deletion of <i>HAT1</i> in <i>S</i>. <i>cerevisiae</i> (a), <i>C</i>. <i>glabrata</i> (b) and <i>S</i>. <i>pombe</i> (c) has no effect on H₂O₂ resistance. Exponentially growing cells were treated with 5 mM (a), 50 mM (b) or 20 mM (c) H₂O₂ for 2 hours. Cells were plated and colonies counted after 3 days of incubation on YPD plates at 30°C to determine viability. Data are shown as mean + SD from three independent experiments. (B) Lack of Hat1 in <i>S</i>. <i>cerevisiae</i> (a) and <i>C</i>. <i>glabrata</i> (b) does not increase azole tolerance. Deletion of Hat1 in <i>S</i>. <i>pombe</i> reduces susceptibility to voriconazole (c). Logarithmically growing cells were diluted into medium containing 150 ng/ml (a), 1000 ng/ml (b) or 800 ng/ml (c) voriconazole and incubated at 30°C for 24 hours. OD₆₀₀ was determined and growth inhibition relative to untreated samples was calculated. Data are shown as mean + SD from three independent experiments. (C) <i>C</i>. <i>parapsilosis</i> (a) and <i>C</i>. <i>tropicalis</i> (b) <i>hat1</i>Δ/Δ cells show increased resistance to H₂O₂. Experiment was performed as described in (A). H₂O₂ concentrations were 50 mM (a) and 20 mM (b). (D) Loss of Hat1 in <i>C</i>. <i>parapsilosis</i> (a) and <i>C</i>. <i>tropicalis</i> (b) reduces susceptibility to voriconazole. Experiment was performed as described in (B). For <i>C</i>. <i>parapsilosis</i> cells were incubated for 41 hours prior to OD₆₀₀ measurement. Voriconazole concentrations were 50 ng/ml (a) and 200 ng/ml (b). (A-D) *P<0.05, **P<0.01 and ***P<0.001 relative to the corresponding wild-type (Student's t-test).