LIF inhibition during placental development alters decidual immune cell activation transcription factors.

<p><b>Representative photomicrographs of</b> F4/80 macrophage immunostaining performed on E10 or E13 implantation sites treated with PEG control or PEGLA from E8-10 or E10-13 respectively <b>(a)</b>. Bars represent 200μm. Inset is the negative control. Arrows denote F4/80-positive immunostained marcrophages. <b>(b)</b> Staining intensity was analysed in 3 mid-sagital implantation site sections per mouse (n = 4/group) and staining area per decidual are calculated and averaged using CellSens software. Data are mean ± SEM. <b>(c)</b> MCP-1, <b>(d)</b> IL-10 and <b>(e)</b> IFN-γ mRNA expression was determined by semi-quantitative PCR normalized to β2-microglobulin in PEG or PEGLA treated mouse decidual tissue. Data are mean ± SEM, students t-test, *p<0.05.