Non-detectability of low-copy <i>HOX</i> transcripts after Whole Transcriptome Amplification (WTA).

<p>Similar to RT-PCR analysis, the same cDNA of the respective human bone marrow-derived multipotent stromal cell (BM-MSC) line (biological replicates, n = 9) synthesized by standard reverse transcription (RT samples) or Whole Transcriptome Amplification (WTA samples) was used for qPCR analysis. (A-C) The number of samples out of the 9 tested samples is shown in brackets, where the respective low-copy <i>HOX</i> transcript could be detected in qPCR analysis. The low-copy <i>HOX</i> transcripts were undetectable in the remaining samples. In comparison to WTA samples, all RT samples of BM-MSC lines revealed <i>HOXA4</i>, <i>HOXD3</i> and <i>HOXD9</i> gene expression.