Biased qPCR results for medium-copy <i>HOX</i> transcripts after Whole Transcriptome Amplification (WTA).

<p>Similar to RT-PCR analysis, the same cDNA of the respective human bone marrow-derived multipotent stromal cell (BM-MSC) line (biological triplicates, n = 3) synthesized by standard reverse transcription (RT samples) and Whole Transcriptome Amplification (WTA samples) was used for qPCR analysis. (A-C) The WTA samples revealed a significantly decreased expression level of the medium-copy <i>HOX</i> genes like <i>HOXA9</i>, <i>HOXB7</i> and <i>HOXD8</i> in comparison to RT samples. (D) The reference gene <i>RPL13A</i> is expressed on a significantly higher level compared to the <i>HOX</i> genes resulting in no significantly different qPCR results after WTA.