Sequence LOGOs analysis.

<p>(<b>A</b>) Sequence LOGOs of the R2 and R3 DNA-recognition α-helices of <i>Arabidopsis thaliana</i> R2R3-MYB DBD subgroups (left) and of the bound <i>cis</i>-elements assayed on the Y1H experiments, classified into two groups (right). The bottom LOGOs are the calculated <i>consensi</i> for both groups. Boxed columns within the recognition helices highlight residues that most likely contact DNA nitrogen bases based on alignments to MYB protein data bank structures. Red arrowheads indicate key amino acid residues involved in the interaction with DNA that are conserved in almost all plant and animal MYB proteins ([<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0141044#pone.0141044.ref050" target="_blank">50</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0141044#pone.0141044.ref054" target="_blank">54</a>]). Red squares highlight key amino acid residues that are associated with specific DBD subgroups and for which some experimental evidences (<i>in vitro</i> and/or <i>in vivo</i>) on their role in the interaction with DNA targets are available. <b>(B)</b><i>AC</i>-rich sequence LOGOs associated with the <i>trans</i>-activation activity of DBD subgroup 1, 2, 3, 13 and 24.