<p>Equal volumes of <i>Agrobacterium</i> cultures carrying the plasmids encoding each of the antigenome derivatives, core proteins and VSRs were mixed and infiltrated into <i>N</i>. <i>benthamiana</i> leaves. Systemically infected plants were confirmed by symptom inspection and RT-PCR. The number of systemically infected per inoculated plants in three independent experiments (Exp.) were used to calculate the total percentage of systemic infectivity.</p><p><sup>a</sup> SYNV antigenomic cDNA and its derivatives were cloned into the pCB301 binary vector. MR-sc4-RFP is an SYNV antigenome minireplicon derivative containing an sc4 gene substitution for the N ORF and a RFP gene substitution for the P ORF.</p><p><sup>b</sup> N: pGD-N; P: pGD-P; L: ...
My thesis projects are related to two plant defense mechanisms: RNA silencing and pathogen-induced d...
<p>(<b>A</b>) Phenotype of <i>Su</i> silencing in the wt and <i>NbRDR6</i> RNAi line (dsRDR6) induce...
Tomato bushy stunt virus (TBSV) is one of few RNA plant viruses capable of moving systemically in so...
<p>(A) Schematic representation of the SYNV infectious cDNA clone and supporting plasmids. The pSYNV...
Reverse genetics systems have been established for all major groups of plant DNA and positive-strand...
<p>(A) Symptoms of <i>N</i>. <i>benthamiana</i> plants after agroinfiltration with rSYNV-GFP and rSY...
<p>(A) The transcriptional levels of <i>NbTOM1</i> were detected in mock inoculation buffer, HLSV, T...
<p>(A) Diagram showing insertion of the GFP gene between the N and P genes of the rSYNV antigenome t...
<p>Whole-plant panels in the left: at seven days post inoculation (dpi), all plants displayed infect...
<p>(A) Schematic representation of full-length cDNA clones derived from PPV and employed to infect <...
<p>(<b>A</b>) The RNaseIII signature motif (blue) and dsRNA-binding domain (red) conserved in Class ...
12 p.-8 fig.Potato Virus X (PVX) is the type member of Potexvirus genus, a group of plant viruses wi...
<p>(<b>A</b>) Gene silencing of <i>NbGAPDH-A</i> was induced in <i>N. benthamiana</i> plants inocula...
<p><i>N</i>. <i>benthamiana</i> 16C plants were infiltrated at leaf L4 with pBinGFP in the additiona...
AbstractDifferent isolates of Alternanthera mosaic virus (AltMV; Potexvirus), including four infecti...
My thesis projects are related to two plant defense mechanisms: RNA silencing and pathogen-induced d...
<p>(<b>A</b>) Phenotype of <i>Su</i> silencing in the wt and <i>NbRDR6</i> RNAi line (dsRDR6) induce...
Tomato bushy stunt virus (TBSV) is one of few RNA plant viruses capable of moving systemically in so...
<p>(A) Schematic representation of the SYNV infectious cDNA clone and supporting plasmids. The pSYNV...
Reverse genetics systems have been established for all major groups of plant DNA and positive-strand...
<p>(A) Symptoms of <i>N</i>. <i>benthamiana</i> plants after agroinfiltration with rSYNV-GFP and rSY...
<p>(A) The transcriptional levels of <i>NbTOM1</i> were detected in mock inoculation buffer, HLSV, T...
<p>(A) Diagram showing insertion of the GFP gene between the N and P genes of the rSYNV antigenome t...
<p>Whole-plant panels in the left: at seven days post inoculation (dpi), all plants displayed infect...
<p>(A) Schematic representation of full-length cDNA clones derived from PPV and employed to infect <...
<p>(<b>A</b>) The RNaseIII signature motif (blue) and dsRNA-binding domain (red) conserved in Class ...
12 p.-8 fig.Potato Virus X (PVX) is the type member of Potexvirus genus, a group of plant viruses wi...
<p>(<b>A</b>) Gene silencing of <i>NbGAPDH-A</i> was induced in <i>N. benthamiana</i> plants inocula...
<p><i>N</i>. <i>benthamiana</i> 16C plants were infiltrated at leaf L4 with pBinGFP in the additiona...
AbstractDifferent isolates of Alternanthera mosaic virus (AltMV; Potexvirus), including four infecti...
My thesis projects are related to two plant defense mechanisms: RNA silencing and pathogen-induced d...
<p>(<b>A</b>) Phenotype of <i>Su</i> silencing in the wt and <i>NbRDR6</i> RNAi line (dsRDR6) induce...
Tomato bushy stunt virus (TBSV) is one of few RNA plant viruses capable of moving systemically in so...