Expression and distribution of HMGB1 in the KA-induced mouse epileptic brain tissues.

<p>(A&B) Immunohistochemical study detecting HMGB1 in the CA1 (A) and CA3 (B) hippocampal regions from the NS group mice (<i>n</i> = 5). HMGB1 immunoreactivity was detected mostly in the nuclei of the pyramidal neurons. (C&D) Immunohistochemical study detecting HMGB1 in the CA1 (C) and CA3 (D) hippocampal regions from the EP group mice (<i>n</i> = 6). Cytoplasmic staining of HMGB1 was substantially increased in the pyramidal neurons compared with the NS group. Glial cells with both nuclear and cytoplasmic staining were also found. Scale bar: 50 μm. Red arrows: neurons with nuclear staining; Blue arrows: glial cells with nuclear staining; Red arrowheads: neurons with both nuclear and cytoplasmic staining; Blue arrowheads: glial cells with both nuclear and cytoplasmic staining. EP, KA-induced epileptic seizure group; HMGB1, high-mobility group box-1; KA, kainic acid; NS, normal saline control group.