HMGB1 had no significant effect on the viability of bEnd.3 cells.

<p>Cells were treated with different concentrations of HMGB1 for the indicated time periods. Cell viability was determined using CCK8 assay. Data from three independent experiments was shown as mean±SD; <i>n</i> = 3. CCK8, cell counting kit; HMGB1, high-mobility group box-1; SD, standard deviation.