Primary inflorescence height of four independent transgenic lines overexpressing the At1g74450 open reading frame.

<p>(A) Graph showing the average primary inflorescence height in cm for four independent 35S:ORF lines compared to wildtype Col-0. Bars indicate standard error for each line (2: n = 31, 3: n = 27, 4: n = 30, 5: n = 20, Col-0: n = 117). The asterisks indicate statistically significant differences (p<0.01) between each 35S:ORF line and wildtype Col-0 based on one-way ANOVA followed by post hoc t-tests (two-tailed distribution, two-sample unequal variance) and Bonferroni correction. (B) Composite gel image showing semi-quantitative RT-PCR results for the four independent 35S:ORF lines and wildtype Col-0. The top half of the gel image shows RT-PCR products after 28 cycles generated by constitutive control Alpha-(α)-Tubulin primers [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140368#pone.0140368.ref021" target="_blank">21</a>]. The lower half of the gel image shows RT-PCR products after 28 cycles generated by gene-specific primers.