Recent enhancements to the functional form of the Rosetta energy function enable improved performance.

<p><b>(A)</b> Rosetta’s “Talaris” energy function [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140359#pone.0140359.ref019" target="_blank">19</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140359#pone.0140359.ref020" target="_blank">20</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140359#pone.0140359.ref030" target="_blank">30</a>] includes updates to the functional form of the hydrogen bond term and of the electrostatic term. These changes lead to improved performance relative to score12, at a statistically significant level (p = 0.013). <b>(B)</b> Replacing Rosetta’s default model of polar solvation, EEF1, with a newly developed model, pwSHO [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140359#pone.0140359.ref031" target="_blank">31</a>], leads to further improvement at a statistically significant level (p = 0.0004).