Target cell populations of FV infection.

<p>C57BL/6 mice were infected with FV and splenocytes were isolated at different time points after infection. Multi-parameter flow cytometry analysis was used to compare the expression of FV gp70 antigen on the cell surface of different subpopulations of spleen cells. <b>A</b>. representative histograms of all nucleated cells positive for gp70 from naïve, 6 day and 10 day infected mice. The bars represent percentages of all nucleated spleen cells positive for gp70 for a group of 6–10 mice. <b>B</b>. The representative dot plots of Ter119⁺ cells positive for gp70 from naïve, 6 day and 10 day infected mice. Numbers in the upper right quadrat represent the percentage of gp70⁺ of Ter119⁺ cells. The bars represent the number of non-infected Ter119⁺ erythroblasts (gp70 negative) per one million nucleated cells (white columns) and the number of infected Ter119⁺gp70⁺ cells (black columns). <b>C</b>. The representative dot plot of CD19⁺ cells positives for gp70 from naive, 6 day and 10 day infected mice. Numbers in the upper right quadrat represent the percentage of gp70⁺ of CD19⁺ cells. The bars represents the number of non-infected CD19⁺ B cells (gp70 negative) per one million nucleated cells (white columns) and the number of infected CD19⁺gp70⁺ cells (black columns). <b>D</b>. The representative dot plot of Gr-1⁺ cells positives for gp70 from naive, 6 day and 10 day infected mice. Numbers in the upper right quadrat represent the percentage of gp70⁺ of Gr-1⁺ cells. The bars represents the number of non-infected myeloid Gr-1⁺ cells (white column) and the number of infected Gr-1⁺ gp70⁺ cells (black columns). <b>E</b>. The frequency of gp70⁺ cells at day 10 post infection in relation to the infected cells at day 6 from C57BL/6 (wt) (white bars) and PD-L1^-/- (black bars) mice. Mean numbers plus SD from experiments with 5–8 mice are shown. Data was pooled from three independent experiments with similar results. Differences between frequencies of infected (gp70⁺) cells from different populations were analyzed by an unpaired t-test and are indicated in the figure (**p˂0.005, ***p˂0.0005).