Gene expression patterns among CD62Lhi memory CD8 T cells change with time after infection.

<p>mRNA was isolated from sorted CD62Lhi <i>early</i>M (30–45 days p.i.) and <i>late</i>M (8+ months p.i.) P14 cells and used for microarray hybridization. (A) Heat map of genes with significantly different mRNA expression between <i>early</i>M and <i>late</i>M CD62Lhi P14 cells with a fold change >1.25. (B) Biological pathway analysis of genes with significant mRNA changes of fold >1.25 in CD62Lhi <i>early</i>M and <i>late</i>M P14 cells was generated using the KEGG pathway tool in DAVID bioinformatics resources. ↑, genes with increased expression in CD62Lhi <i>late</i>M compared to <i>early</i>M P14 cells. ↓, genes with decreased expression in CD62Lhi <i>late</i>M compared to <i>early</i>M P14 cells. Data were generated from six separate microarray hybridizations performed using mRNA extracted from sorted <i>early</i>M and <i>late</i>M P14 cells from three individual mice per group.