Proliferation and ‘memory generation potential’ of CD62Lhi memory cells increases with time after infection.

<p>Sorted CD62Lhi Thy disparate <i>early</i>M and <i>late</i>M P14 cells were mixed and injected into naïve recipients followed by i.p. injection of LCMV Armstrong 24 hours later. (A) Dot plot showing the master mix of CD62Lhi <i>early</i>M (Thy1.1/1.1) and <i>late</i>M (Thy1.1/1.2) P14 cells used for adoptive transfer. (B) Representative plot showing the response of progeny of sorted CD62Lhi <i>early</i>M (Thy1.1/1.1) and <i>late</i>M (Thy1.1/1.2) P14 cells in PBL d8 after LCMV infection. (C) Kinetic analysis of secondary responses generated from sorted CD62Lhi <i>early</i>M (open squares) and <i>late</i>M (black squares) P14 cells in PBL at the indicated days post LCMV infection. (D) Ratio of cells generated from sorted CD62Lhi <i>late</i>M to <i>early</i>M P14 cells in PBL at the indicated days post LCMV infection. Doted line indicates the starting ratio of CD62Lhi <i>late</i>M to <i>early</i>M P14 cells. (E) Ratio of cells generated from sorted CD62Lhi <i>late</i>M to <i>early</i>M P14 cells in the indicated organs at a memory time point (d140) following LCMV infection. Doted line indicates the starting ratio of CD62Lhi <i>late</i>M to <i>early</i>M P14 cells. R:, ratio of CD62Lhi <i>late</i>M to <i>early</i>M P14 cells or progeny of CD62Lhi <i>late</i>M to <i>early</i>M P14 cells.*, statistically significant (p<0.05) as determined by Student t-test. Representative data from one of two individual experiments with 5 mice per group per experiment. Error bars represent the standard error of the mean.