Additional file 4: Figure S4. of The kinase inhibitor D11 induces caspase-mediated cell death in cancer cells resistant to chemotherapeutic treatment

Western blot analysis of NF-κB protein expression and phosphorylation levels. Whole cell extracts from U-87 MG cells treated with vehicle or 50 μM D11 for 5 h and stimulated with 10 ng/ml TNFα for 10 min prior to harvesting, were analyzed by immunoblotting employing antibodies against NF-κB protein or the phosphorylated form at S536 and S529, respectively. β-actin detection was used as loading control. (JPEG 26 kb