<i>Yersinia pestis</i> Requires Host Rab1b for Survival in Macrophages

<div><p><i>Yersinia pestis</i> is a facultative intracellular pathogen that causes the disease known as plague. During infection of macrophages <i>Y</i>. <i>pestis</i> actively evades the normal phagosomal maturation pathway to establish a replicative niche within the cell. However, the mechanisms used by <i>Y</i>. <i>pestis</i> to subvert killing by the macrophage are unknown. Host Rab GTPases are central mediators of vesicular trafficking and are commonly targeted by bacterial pathogens to alter phagosome maturation and killing by macrophages. Here we demonstrate for the first time that host Rab1b is required for <i>Y</i>. <i>pestis</i> to effectively evade killing by macrophages. We also show that Rab1b is specifically recruited to the <i>Yersinia</i> containing vacuole (YCV) and that <i>Y</i>. <i>pestis</i> is unable to subvert YCV acidification when Rab1b expression is knocked down in macrophages. Furthermore, Rab1b knockdown also altered the frequency of association between the YCV with the lysosomal marker Lamp1, suggesting that Rab1b recruitment to the YCV directly inhibits phagosome maturation. Finally, we show that Rab1b knockdown also impacts the pH of the <i>Legionella pneumophila</i> containing vacuole, another pathogen that recruits Rab1b to its vacuole. Together these data identify a novel role for Rab1b in the subversion of phagosome maturation by intracellular pathogens and suggest that recruitment of Rab1b to the pathogen containing vacuole may be a conserved mechanism to control vacuole pH.</p>