Inhibited growth of Hela cells at 39°C.

<p><b>Left panel</b>: Hela cells usually grow in three dimensions in the dish to form spherical colonies which, after growing beyond 9 days, easily shed off during crystal violet staining, leaving the dish with only the periphery of the colonies, especially in the 37°C dish wherein the colonies are much larger. <b>Right panel</b>: Six days after the cells were seeded, individual cells seeded at a density of about 20,000 cells per 35-mm dish develop to larger colonies, compared with the colonies in the dish wherein cells were seeded at a density of 2,000 cells. Cells at 37°C develop larger colonies than at 39°C.