The downregulation of Chk-α attenuated the phosphorylation levels of ERK1/2, AKT and mTOR.

<p>(A) Western blot analysis of the phosphorylation of AKT. (B) Western blot analysis of the phosphorylation of ERK1/2 (C) Western blot analysis of the phosphorylation of mTOR. The phoshorylation levels of AKT, ERK1/2 and mTOR decreased MCF-7-shChk-α and MCF-7/TAM-shChk-α cells relative to MCF-7 and MCF-7/TAM cells. Data are presented as the mean ± standard deviation of 5 independent experiments. *<i>p</i><0.05. shChk-α transduced vs control.