Characterisation of α2β3γ2L GABA_ARs expressed at a 3:1:3 injection ratio.

<p>(A) <i>Left panel</i>, Representative traces of α2β3γ2L (3:1:3) GABA_ARs responses to 3 mM GABA and 100 μM Zn²⁺ alone. <i>Right panel</i>, Mean holding current of oocytes expressing α2β3γ2L (3:1:3) GABA_ARs (-93 ± 19 nA; <i>n</i> = 8). (B) <i>Left panel</i>, Continuous traces demonstrating two consecutive applications of control (100 μM GABA) followed by the co-application of 10 μM Zn²⁺ with control. <i>Right panel</i>, Modulation of 100 μM GABA responses by 10 μM Zn²⁺ (<i>n</i> = 9). (C) <i>Left panel</i>, Continuous traces demonstrating two consecutive applications of control (1 μM GABA) followed by the co-application of 1 μM diazepam with control; 1 μM diazepam and 10 μM flumazenil with control; and control. <i>Right panel</i>, Potentiation by 1 μM diazepam of 1 μM GABA responses (<i>n</i> = 6). Representative traces demonstrating (D) GABA current responses from 1 μM to 10 mM and (E) 2’MeO6MF’s direct activation from 1 to 300 μM (red) in comparison to 3 mM GABA response. Concentration-response curves of GABA (black; <i>n</i> = 6) and 2’MeO6MF (red; <i>n</i> = 5) are shown in (F). Data are presented as mean ± SEM. Bars indicate durations of drug application. The holding current values are represented by the dotted lines.