Expression analysis of the viral packaging components.

<p>A. Quantitation of the viral gene DNAs in 293T packaging cells. VSVG: the viral packaging gene that encodes of the vesicular stomatitis virus G glycoprotein; GAG: the HIV-1 gene that encodes the P24 non-glycosylated capsid protein CA; REV: the HIV-1 gene that encodes glycosylated transmembrane envelope protein; POL: the HIV-1 gene that encodes the RNA polymerase; mCherry: the vector control. All data shown are mean±SEM from three independent experiments by normalization over controls. B. Expression of viral package genes. Total RNA was extracted 48 hours after viral packaging in 293T cells and was used to analyze the expression of viral packaging genes. β-ACTIN was used the PCR control. ** p<0.01 as compared with normal controls. C. Production of HIV-1 by HIV-1 p24 antigen capture assay. The core of the HIV-1 virion is made up of two strands of RNA and various proteins including the p24 core antigen. The HIV-1 p24 antigen capture assay is a double-antibody sandwich enzyme immunoassay used to quantitate HIV-1 p24 in tissue culture samples. HIV-1 was quantitated 48h after viral packaging in 293T cells. ** p<0.01 as compared with normal controls.