HGF and EGF phosphorylate MET and EGFR, respectively.

<p><b>A-B</b> Immunoblots, showing the effects of <b>A</b> HGF stimulation (0-100ng/ml) in combination with crizotinib on MET (MET: 145kDa, pro-MET: 170kDa) phosphorylation and <b>B</b> EGF stimulation (0-100ng/ml) in combination with canertinib on EGFR (175kDa) phosphorylation in cell lines RES256 and UW473. Bands of MET represent MET (145kDa) and pro-MET (170kDa). Total-MET and total-EGFR were used as loading controls. <b>C-D</b> Quantification analysis of immunoblots showing the <b>C</b> normalized phospho-MET expression and <b>D</b> normalized phospho-EGFR expression. Quantitations are representative of 2 to 3 independent experiments. Student’s t-test: *p<0.05. <b>E-F</b> Quantitative analysis of the effects of <b>E</b> HGF and <b>F</b> EGF using human phospho-kinase arrays in cell lines RES256 and UW473. The plot displays the phosphorylation sites of the proteins present on the proteome array that are categorized upon different signal transduction pathways. Mean pixel intensities were calculated and subtracted by the mean background intensity of the array. Bars depict the difference between growth factor-stimulated cells and control cells. Positive values indicate growth factor-induced phosphorylation of the kinase.