HENMT1 is required for the repression of <i>Line-1</i> and <i>IAP</i> retrotransposons in spermatocytes and round spermatids.

<p><b>(A-D)</b> qPCR analyses of (<b>A</b>) <i>L1_A</i> (<b>B</b>) <i>L1_TF14</i> (<b>C</b>) <i>IAP_LTR</i> (<b>D</b>) <i>IAP_GAG</i> in <i>Henmt1</i>^<i>WT/WT</i> and <i>Henmt1</i>^{<i>PIN/PIN</i>} spermatocytes and round spermatids (round) purified from 30 day old mice. A two-tailed unpaired student T-test was performed for statistical analysis, n = 5/genotype, +/- SEM, * p<0.05; ** p<0.01; ***p<0.001; ****p<0.0001. <b>(E-F)</b> RNA <i>in situ</i> hybridisation for <i>Line-1</i> expression in <i>Henmt1</i>^<i>WT/WT</i> and <i>Henmt1</i>^{<i>PIN/PIN</i>} 10 weeks-old testes. Black arrows indicate pachytene spermatocytes. Red arrows indicate round spermatids. <b>(G)</b> Staining for LINE-1 in in <i>Henmt1</i>^<i>WT/WT</i> and <i>Henmt1</i>^{<i>PIN/PIN</i>} in 10 weeks-old testes. The boxed areas are magnified in the panel to the immediate right of each genotype. The scale bar = 50 μm. (<b>H</b>) Immunofluorescence staining for γH2AX (red) as a marker of DNA double stranded break respectively in <i>Henmt1</i>^<i>WT/WT</i> and <i>Henmt1</i>^{<i>PIN/PIN</i>} in 10 weeks-old testes. The white arrow indicates elevated γH2AX staining in spermatids compared to wild type cells. The boxed areas are magnified in the panel to the immediate right of each genotype. The scale bar = 50 μm.