HENMT1 is required for mouse spermatogenesis.

<p><b>(A-D)</b> Periodic acid-Schiff (PAS) stained testis sections from <i>Henmt1</i>^<i>WT/WT</i> and <i>Henmt1</i>^{<i>PIN/PIN</i>} mice. Arrows indicate the presence of pinhead-shaped sperm heads. Arrow heads indicate symplasts composed of coalesced germ cells. <b>(E)</b> Testis weight in <i>Henmt1</i>^<i>WT/WT</i> and <i>Henmt1</i>^{<i>PIN/PIN</i>} mice (n = 6 / genotype +/- SEM, * p<0.05). <b>(F)</b> Daily sperm production in <i>Henmt1</i>^<i>WT/WT</i> and <i>Henmt1</i>^{<i>PIN/PIN</i>} mice expressed as percentage of <i>Henmt1</i>^<i>WT/WT</i> (n = 6 / genotype +/- SEM, ** p<0.01) <b>(G-H)</b> PAS stained sections of epididymides from <i>Henmt1</i>^<i>WT/WT</i> and <i>Henmt1</i>^{<i>PIN/PIN</i>} mice. <b>(I)</b> Epididymal sperm content in <i>Henmt1</i>^<i>WT/WT</i> and <i>Henmt1</i>^{<i>PIN/PIN</i>} mice expressed as percentage of <i>Henmt1</i>^<i>WT/WT</i> (n = 3 / genotype +/- SD, ****p<0.0001). <b>(J)</b> Hematoxylin and eosin stained sperm from <i>Henmt1</i>^<i>WT/WT</i> and <i>Henmt1</i>^{<i>PIN/PIN</i>} mice showing the pin-shaped heads and the absence of a mitochondrial sheath (open arrow) in <i>Henmt1</i>^{<i>PIN/PIN</i>} sperm. <b>(K)</b> The percentage of sperm showing any forms of motility in <i>Henmt1</i>^<i>WT/WT</i> and <i>Henmt1</i>^{<i>PIN/PIN</i>} mice (n = 3 / genotype +/- SD, ****p<0.0001). <b>(L)</b> The percentage of sperm showing progressive motility in <i>Henmt1</i>^<i>WT/WT</i> and <i>Henmt1</i>^{<i>PIN/PIN</i>} mice (n = 3 / genotype +/- SD, ****p<0.001). <b>(M-N)</b> Electron microscopy of stage VIII pachytene spermatocytes showing the presence of inter-mitochondrial cement (arrow) and the chromatoid body (red square) in <i>Henmt1</i>^<i>WT/WT</i> and <i>Henmt1</i>^{<i>PIN/PIN</i>} mice. All data was collected from 10 week-old mice. A two-tailed unpaired student T-test was performed for statistical analyses.