Additional file 5: of The strigolactone biosynthesis gene DWARF27 is co-opted in rhizobium symbiosis

Nodule phenotype after knock-down of MtD27 through RNAi. (a) Relative transcript abundance as determined by qRT-PCR of MtD27 in M. truncatula transgenic roots expressing an empty vector control construct (EVi) or MtD27 RNAi construct (D27i). (b) Number of nodules formed on plants bearing transgenic roots harboring an empty vector control construct (EVi) or MtD27 RNAi construct (D27i). (c) Section through a nodule formed on a root expressing the empty vector control construct. (d) Section through a nodule formed on a root expressing the MtD27 RNAi construct. Scale bars are equal to 100 μm. Data shown in (a,b) represent means of 6 (a) or 5 (b) biological replicates ± SEM. Different letters above bars indicate statistical difference (p < 0.05, students’ t-test). (TIFF 4222 kb