Antibody-opsonized virions have exposed genomes and colocalize with cGAS inside infected cells.

<p>(<b>A</b>) Confocal microscope image of MEF cells 2 hours post infection with AdV containing BrdU-labeled DNA (BrdU-AdV), stained with DAPI and anti-BrdU antibody. Scale bars show 20 μm. (<b>B</b>) As (A) except BrdU-AdV pre-incubated with wt9C12. (<b>C</b>) As (A), except BrdU-AdV pre-incubated with mut9C12. (<b>D</b>) BrdU-positive puncta per cell from confocal images from conditions G-I. *** p < 0.001, n ≥ 100 cells per condition. (<b>E</b>) As (B), except HeLa cells transfected with FLAG-cGAS prior to infection, and also stained with anti-FLAG antibody. Scale bars show 20 μm.