Frequencies of circulating regulatory T-cells in PBMC following <i>T</i>. <i>solium</i> antigen stimulation.

<p>(A) Representative plots showing fluorescence for the isotype control mAbs for Tregs-specific mAbs in lymphocytes, and expression of CD25, CD127 and FoxP3 in unstimulated and TS Ag-stimulated cells (B) Gating strategy for Treg analysis in PBMC from NCC patient. Cells defined by the lymphocytes gate were analyzed for CD4 and CD25 expression, and CD4/CD25 double positive cells were analyzed for expression of CD127 and FoxP3 to identify Treg (CD127^low/-FoxP3⁺) cells and (C) Regulatory T cells in each study group enumerated by flow cytometry after culture with medium alone or <i>T</i>. <i>solium</i> antigen (20 and 40 μg/ml) on day 3. Data presented are the mean ± SEM of NCC patients with parenchymal (n = 16) and subarachnoid disease (n = 13). * indicate statistically significant differences (p<0.05) between the three groups compared pairwise with medium alone by Mann Whitney U test.