C-di-GMP binds MshE and PA14_29490 in the N-terminal T2SSE_N domain.

<p>Schematic of the truncations generated in (A) PA14_29490 and (E) MshE. C-di-GMP binding to <i>E</i>. <i>coli</i> whole cell lysates expressing each fragment of (B) PA14_29490 and (F) MshE. (C) Fraction bound ³²P-c-di-GMP to decreasing concentrations of purified PA14_29490 fragment 1. (D) Fraction bound of purified PA14_29490 fragment 1 to ³²P-c-di-GMP in the presence of 100 μM nucleotide competitors. (G) Fraction bound of ³²P-c-di-GMP to purified MshE and fragments 1–6. Indicated p-value was determined by two-tailed t-test (*** p≤0.001) by comparing the indicated samples. All data are average of three independent assays and standard deviation is indicated by error bars.