Unc93b is cleaved by 3C^pro during CVB infection.

<p><b>(a)</b> 293T cells transfected with EGFP-Unc93b were infected with CVB (MOI = 1), lysates harvested 18 hours post-infection, then subjected to immunoblotting with anti-GFP, -VP1, and -GAPDH antibodies. (<b>b</b>) 293T cells transfected with EGFP-Unc93b were infected with CVB or UV-inactivated CVB (MOI = 1), lysates harvested 18 hours post-infection, then subjected to immunoblotting with anti-GFP, -VP1, and -GAPDH antibodies. <b>(c)</b> 293T cells were cotransfected with the indicated EGFP-fused Unc93b construct and Myc-tagged wild-type (WT) or catalytically inactive C147A 3C^pro, lysates were harvested 48 hours post-transfection and subjected to immunoblotting with anti-GFP and -GAPDH antibodies. (<b>d</b>) 293T cells transfected with the indicated EGFP-fused Unc93b construct were infected with CVB (MOI = 1), lysates were harvested 16 hours post-infection and subjected to immunoblotting with anti-GFP, -VP1, and–GAPDH antibodies. In all panels, ns denotes nonspecific bands.