The expanded piriform cortex showed no obvious lamination defects in E18.5 <i>Gli3</i>^<i>cKO</i> mutants.

<p><b>(A)</b> Ctip2 labels neocortical neurons in neocortical layer V and layer II neurons in the piriform cortex in control brains. CR labels mitral cell axons comprising the LOT. <b>(B)</b> Higher magnification revealing the position of Ctip2+ layer II neurons with respect to the CR+ axons of the LOT. <b>(C-D)</b> In <i>Gli3</i>^<i>cKO</i> mutants, Ctip2+ cells occupy layer II in the piriform cortex below the CR+ axons. <b>(E, F)</b> Organization of the piriform cortex visualized by immunofluorescence analysis for CR, Map2 and Dapi. CR labels the LOT axons; Map2 identifies dendrites in layer I and the dense population of cells in layer II is stained with Dapi. <b>(G, H)</b> In <i>Gli3</i>^<i>cKO</i> mutants, no obvious defects are observed in piriform cortex organization. Abbreviations: ctx, neocortex; LOT, lateral olfactory tract; pc, piriform cortex. Arrows in A, D, E and H indicate the rhinal fissure and the transition from neocortex to piriform cortex. Scale bars: A-H:250μm.