Characterization of CD14⁺CD169⁺IL-10⁺ M2 cells in CRC patients.

<p>PBMCs and TIMs were isolated from individual subjects and stimulated with LPS and PMA/ionomycin. The cells were stained with anti-CD169 and anti-CD14 for 30 min, fixed, and permeabilized, followed by intracellular staining with anti-IL-10 or anti-IL-12. The cells were first gated on CD14⁺CD169⁺ cells and the percentages of CD14⁺CD169⁺IL-12⁺ M1 and CD14⁺CD169⁺IL-10⁺ M2 cells in PBMCs or TIMs from individual subjects were determined by flow cytometry. The levels of plasma IL-10 and IL-12 in individual subjects were determined by ELISA. Data are representative charts or expressed as the values of individual patients. The horizontal lines indicate the median for individual groups. (A) Flow cytometry analysis. (B) The percentages of CD14⁺CD169⁺IL-10⁺ M2 cells. (C) The percentages of CD14⁺CD169⁺IL-12⁺ M1 cells. The horizontal lines indicate the median values. (D) The potential association between the percentages of circulating CD14⁺CD169⁺IL-10⁺ cells and CD14⁺CD169⁺ cells in CRC patients. (E) The potential association between the percentages of TIMs CD14⁺CD169⁺IL-10⁺ cells and CD14⁺CD169⁺cells in the CRC tissues. (F) The levels of plasma IL-10. (G) The levels of plasma IL-12. (H) The correlation of plasma IL-10 levels with the percentages of CD14⁺CD169⁺ IL-10⁺ monocytes in PBMCs from CRC patients. (I) The correlation between the levels of plasma IL-10 and CEA in CRC patients.